Enhanced level of micronuclei in peripheral blood lymphocytes of patients treated for restenosis with 32P endovascular brachytherapy

Wojcik, Andrzej; Buraczewska, Iwona; Sommer, Sylwester; Brzozowska, Kinga; Pręgowski, Jerzy; Witkowski, Adam; Garmol, Dariusz; Pszona, Stanisƚaw; Bulski, Wojciech

doi:10.1016/j.carrev.2007.07.005

« Previous Next »Cardiovascular Revascularization Medicine
Volume 9, Issue 3 , Pages 149-155, July 2008

Enhanced level of micronuclei in peripheral blood lymphocytes of patients treated for restenosis with ³²P endovascular brachytherapy☆

Andrzej Wojcik
- Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
- Department of Radiobiology and Immunology, Institute of Biology, Swietokrzyska Academy, Kielce, Poland
- Corresponding author. Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, ul. Dorodna 16, 03-195 Warsaw, Poland. Tel.: +48 22 5041228; fax: +48 22 8111532.
Iwona Buraczewska
- Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
Sylwester Sommer
- Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
Kinga Brzozowska
- Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
Jerzy Pręgowski
- Cardiac Catheterization Laboratory, Institute of Cardiology, Warsaw, Poland
Adam Witkowski
- Cardiac Catheterization Laboratory, Institute of Cardiology, Warsaw, Poland
Dariusz Garmol
- Department of Medical Physics, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
Stanisƚaw Pszona
- Soltan Institute for Nuclear Studies, Otwock-Swierk, Poland
Wojciech Bulski
- Department of Medical Physics, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland

Received 5 June 2007; received in revised form 19 July 2007; accepted 24 July 2007.

Abstract

Background

Restenosis is the complete occlusion of the blood vessel leading to such complications as ischemia/angina, myocardial infarction, and death. It can be managed by endovascular brachytherapy with both gamma and beta sources. Endovascular brachytherapy is performed worldwide on several thousands of cases per year. The gamma-emitter ¹⁹²Ir as well as the beta-emitters ³²P and ⁹⁰Sr are mainly used. The dose to the occluded endothelial wall is 20 Gy. Interestingly, no information with respect to the dose absorbed by the blood during the course of the treatment exists. The aim of the present investigation was to verify if the micronucleus test is suitable to detect the dose absorbed by lymphocytes in the course of endovascular brachytherapy with ³²P.

Materials and methods

Blood was drawn from 16 patients immediately before and 1 day after the treatment. Frequencies of micronuclei were assessed. In order to ensure that the micronuclei did not arise due to fluoroscopy or reperfusion, we analyzed lymphocytes of 16 control patients who underwent interventional cardiology with balloon angioplasty only.

Results and conclusions

Enhanced frequencies of micronuclei were observed in lymphocytes of some donors following brachytherapy. No correlation could be detected between the level of induced micronuclei and the absorbed dose. Also, no effect of fluoroscopy or reperfusion was seen. Thus, although brachytherapy of restenosis with ³²P leads to weak enhancement of the micronucleus frequency in lymphocytes, the effect was not seen in all patients; the reason for this heterogeneous response remains to be elucidated.

Keywords: Restenosis, Angioplasty, Brachytherapy, Lymphocytes, Micronuclei