Cardiovascular Revascularization Medicine
Volume 9, Issue 3 , Pages 149-155 , July 2008

Enhanced level of micronuclei in peripheral blood lymphocytes of patients treated for restenosis with 32P endovascular brachytherapy

  • Andrzej Wojcik

      Affiliations

    • Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
    • Department of Radiobiology and Immunology, Institute of Biology, Swietokrzyska Academy, Kielce, Poland
    • Corresponding Author InformationCorresponding author. Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, ul. Dorodna 16, 03-195 Warsaw, Poland. Tel.: +48 22 5041228; fax: +48 22 8111532.
    • ,
  • Iwona Buraczewska

      Affiliations

    • Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
    • ,
  • Sylwester Sommer

      Affiliations

    • Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
    • ,
  • Kinga Brzozowska

      Affiliations

    • Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland
    • ,
  • Jerzy Pręgowski

      Affiliations

    • Cardiac Catheterization Laboratory, Institute of Cardiology, Warsaw, Poland
    • ,
  • Adam Witkowski

      Affiliations

    • Cardiac Catheterization Laboratory, Institute of Cardiology, Warsaw, Poland
    • ,
  • Dariusz Garmol

      Affiliations

    • Department of Medical Physics, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
    • ,
  • Stanisƚaw Pszona

      Affiliations

    • Soltan Institute for Nuclear Studies, Otwock-Swierk, Poland
    • ,
  • Wojciech Bulski

      Affiliations

    • Department of Medical Physics, The Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland

Received 5 June 2007 ,Revised 19 July 2007 ,Accepted 24 July 2007.

References 

  1. Holmes DR. In-stent restenosis. Rev Cardiovasc Med. 2001;2:115–119
  2. Barlis P, Horrigan MC, Chan RK, Ajani AE, Proimos G, Schumer W, et al. What is the best contemporary treatment for in-stent restenosis?. Cardiovasc Revasc Med. 2005;6:179–181
  3. Kirisits C, Hefner A, Wexberg P, Pokrajac B, Glogar D, Potter R, et al. Estimation of doses to personnel and patients during endovascular brachytherapy applications. Radiat Prot Dosimetry. 2004;108:237–245
  4. Dimmick LC, Bass B, Waksman R, Moscovitch M. Estimation of radiation dose received during treatment of in-stent restenosis using ionizing radiation. Cardiovasc Revasc Med. 2005;6:24–31
  5. IAEA . Cytogenetic analysis for radiation dose assessment: a manual. Vienna: IAEA; 2001;
  6. Müller W-U, Streffer C. Micronucleus assays. Adv Mutagen Res. 1994;5:1–134
  7. Fenech M. The in vitro micronucleus technique. Mutat Res. 2000;455:81–95
  8. Sasaki MS. Use of lymphocyte chromosome aberrations in biological dosimetry: possibilities and limitations. In:  Ishihara T,  Sasaki MS editor. Radiation-induced chromosome damage in man. New York: Alan R. Liss, Inc; 1983;p. 585–604
  9. Geise RA, O'Dea TJ. Radiation dose in interventional fluoroscopic procedures. Appl Radiat Isot. 1999;50:173–184
  10. Zakeri F, Assaei RG. Cytogenetic monitoring of personnel working in angiocardiography laboratories in Iran hospitals. Mutat Res. 2004;562:1–9
  11. Zweier JL, Flaherty JT, Weisfeldt ML. Direct measurement of free radical generation following reperfusion of ischemic myocardium. Proc Natl Acad Sci U S A. 1987;84:1404–1407
  12. Andreassi MG, Botto N, Rizza A, Colombo MG, Palmieri C, Berti S, et al. Deoxyribonucleic acid damage in human lymphocytes after percutaneous transluminal coronary angioplasty. J Am Coll Cardiol. 2002;40:862–868
  13. Witkowski A, Pregowski J, Mintz GS, Kalinczuk L, Lyczek J, Kawczynska M, et al. Angiographic restenosis following intravascular beta-brachytherapy does not correlate with delivered dose: a study with dose volume histograms. Recurrence of in-stent restenosis after brachytherapy. Cardiovasc Radiat Med. 2006;4:192–197
  14. Kryscio A, Müller W-U, Wojcik A, Kotschy N, Grobelny S, Streffer C. A cytogenetic analysis of the long-term effect of uranium mining on peripheral lymphocytes using the micronucleus-centromere assay. Int J Radiat Biol. 2001;77:1087–1093
  15. Sachs L. Angewandte Statistik. 6th ed.. Berlin, Heidelberg, New York: Springer Verlag; 1984;
  16. Papworth DG. Exact test of fit for a Poisson distribution. Computing. 1983;31:33–45
  17. Bender MA, Awa AA, Brooks AL, Evans HJ, Groer PG, Littlefield LG, et al. Current status of cytogenetic procedures to detect and quantify previous exposures to radiation. Mutat Res. 1988;196:103–159
  18. Wojcik A, Gregoire E, Hayata I, Roy L, Sommer S, Stephan G, et al. Cytogenetic damage in lymphocytes for the purpose of dose reconstruction: a review of 3 recent radiation accidents. Cytogenet Genet Res. 2004;104:200–205
  19. Stephan G, Kampen WU, Nosske D, Roos H. Chromosomal aberrations in peripheral lymphocytes of patients treated with radium-224 for ankylosing spondylitis. Radiat Environ Biophys. 2005;44:23–28
  20. Vral A, Thierens H, Ridder de L. In vitro micronucleus-centromere assay to detect radiation-damage induced by low doses in human lymphocytes. Int J Radiat Biol. 1997;71:61–68
  21. Norman A, Cochran ST, Sayre JW. Meta-analysis of increases in micronuclei in peripheral blood lymphocytes after angiography or excretory urography. Radiat Res. 2001;155:740–743
  22. Wojcik A, Kowalska M, Bouzyk E, Buraczewska I, Kobialko G, Jarocewicz N, et al. Validation of the micronucleus-centromere assay for biological dosimetry. Genet Mol Biol. 2000;23:25–30
  23. Tamura H, Sugiyama Y, Sugahara T. Changes in the number of circulating lymphocytes with chromosomal aberrations following a single exposure of the pelvis to gamma-irradiation in cancer patients. Radiat Res. 1974;59:653–657
  24. Edwards AA, Lloyd DC, Purrot RJ. Radiation induced chromosome aberrations and the Poisson distribution. Radiat Environ Biophys. 1979;16:89–100
  25. Padjas A, Lesisz D, Lankoff A, Banasik A, Lisowska H, Bakalarz R, et al. Cytogenetic damage in lymphocytes of patients undergoing therapy for small cell lung cancer and ovarian carcinoma. Toxicol Appl Pharmacol. 2005;209:183–191
  26. Banasik A, Lankoff A, Piskulak A, Adamowska K, Lisowska H, Wojcik A. Aluminium-induced micronuclei and apoptosis in human peripheral blood lymphocytes treated during different phases of the cell cycle. Environ Toxicol. 2005;20:402–406

☆ This work was supported by a statutory grant from the Polish Ministry of Science awarded to the Institute of Nuclear Chemistry and Technology.

PII: S1553-8389(07)00261-8

doi: 10.1016/j.carrev.2007.07.005

Cardiovascular Revascularization Medicine
Volume 9, Issue 3 , Pages 149-155 , July 2008

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